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Catalytical degradation of industrial dyes using biosynthesized selenium nanoparticles and evaluating its antimicrobial activities


The release of textile dyes from the industries to the environment has become a significant health issue to humans and their surroundings, where a large number of dyes are wasted to the nearby water during the dyeing process. Therefore, the practice of the utilization of biosynthesized nanoparticles from numerous biological systems has been described, out of which plant material is considered the most appropriate method. The usage of plant material not only makes the procedure ecological but also their abundance makes it more cost-effective. The present study aims to biologically produce selenium nanoparticle using Mucuna pruriens seed powder and apply it in the degradation of dyes and removal of pathogenic bacterial cultures from pathogen-containing lake water using an optimum concentration of selenium nanoparticles. The production of selenium nanoparticles was characterized via visual coloration from colorless to brown solution which was checked using UV–Visible spectrophotometer, the crystalline structure was analyzed using X-ray diffraction, the morphology was analyzed with transmission electron microscopy, scanning electron microscopy, dynamic light scattering, which was in the range of 90–120 nm, with stable spherical monodisperse characteristics. The surface potential was checked using zeta potential, while the Atomic Force Microscopy gave information on the roughness of the nanoparticles, and the presence of –OH, −COO, aromatic groups were confirmed with Fourier Transform Infrared spectroscopy. The biosynthesized nanoparticles were then analyzed for its environmental applications, like degradation of dyes from industrial wastes and checking its antimicrobial activities.


The recent developments in the field of nanoscience have garnered significant attention from various sectors since they can be utilized for detection, diagnostics, purifying industrial waste [1], role in energy production or utilization, catalysis [2], food technology [3] and therapeutics [4]. Nowadays water treatment has become one of the most worrying issues due to upsurge in the industrialization and population that result in contaminating the water resources like the groundwater and reservoir. Therefore it is important to sanitize and reuse municipal and industrial wastewater. In the recent decade, the utilization of nanoparticles for wastewater treatment has picked up the extraordinary consideration because of its property being profoundly gainful as adsorbents and for utilizing for filtration reason [5].

Selenium is vital for proper nourishment in many living organisms, along with humans and other animals, but can be toxic in more significant quantities. Traditional supplements of selenium have a low level of absorption and expanded lethality. Consequently, to create novel materials as transporters of selenium compounds, which would raise the bioavailability of this component and permit its controlled release in the organism, has become a target to many researchers. It is a critical micronutrient and a metalloid (either metal or non-metal), and its supplementation gives various medical advantages. Selenium has several uses like decolorizing glass, making of pigment China Red, applications in laser printers, photocells, photocopiers, semiconductors, steels, and medicinal formulations. Selenium supplementation during fermentation with sugar beet molasses is found to be helpful in the production of fuels like bioethanol, which are clean and cost-effective in comparison to the alternate options [6,7,8,9,10,11].

Dyes are a collection of the synthetic organic complexes that are utilized as colorants in, textile, leather, pharmaceutical paper, cosmetic, food, and plastic industries [12]. Industrial effluents containing dyes are forming a severe environmental threat, as disposal of these effluents into natural water bodies often leads to environmental contamination, serving as a source of non- aesthetic pollution, eutrophication and thereby affecting the aquatic life. The removal or degradation of recalcitrant synthetic dyes is a crucial ecological problem and poses a challenge. Different methods like electrolysis, adsorption, oxidation, coagulation, active sludge biochemical procedures, membrane filtration, ozonization as well as bio-degradation have been used for the elimination of synthetic colorants from the wastewater. But, these recognized technologies are incapable to restrain the contaminant absorption to the preferred level with efficacy, a more efficient yet environment-friendly technique needs to be applied. The photocatalytic action of selenium nanoparticles (SeNPs) to the decolorization of sunset yellow azo-dye and its activity against human cancers has been established in the past already and thus this property was utilized to degrade dyes. In previous studies, it is evident that the photocatalytic analysis of biosynthesized SeNPs has proficiently degraded methylene blue under irradiation through sunlight. Therefore, they can discover applications in wastewater treatment plants and textile enterprises. Subsequently, the mixture of phyto-compounds and nanoparticles serves a functioning role in further pharmacological applications [13,14,15,16].

The fact that they demonstrate biological activity like the utilization as an antimicrobial agent inferable from their interaction with the proteins and different biomolecules present in microbes containing functional groups, for example, −NH, C = O, COO and C-N. The antimicrobial activity can also be contributed due to the presence of phytochemicals surface decorated on the SeNPs like glycoside, alkaloids, flavonoids, steroids, tannin, proteins or tripenoids [17]. The advantages of green synthesis of nanoparticles are the single-step reaction that requires low energy for the initiation of the synthesis and has low incubation period when compared with microorganism based synthesis, it involves usage of fewer chemical which can be hazardous to the environment and is, therefore, cost-effective. The mechanism of nanoparticles synthesized from plant sources pursues the phases like (1) activation phase which involves the breakdown of precursor to elemental forms, (2) nucleation or growth phase of the elemental forms of Se0 (Ostwald ripening), and (3) termination or decline phase that defines the final morphology of the nanoparticles [18, 19]. In our research, we formulated a way to synthesize SeNPs from seed samples in the laboratory on a small scale. In our study we aim to deal with two main aspects using SeNPs: a) Anti-microbial activity to destroy microbes and other harmful organisms in samples taken from water bodies; and b) Dye degradation. Se nanospheres have low toxicity and high biological potentiality this makes them harmless and suitable for addition in water and other natural samples which need to be obtained in a pure form [20].

Materials and methods

Sodium selenite, hydrogen peroxide was purchased from Hi-Media, India. Seeds of Mucuna pureins were purchased from a local drug store in Tamil Nadu. Escherichia coli was purchased from Microbial Type Culture Collection, India. The water sample was collected from the Vellore Institute of Technology (VIT) Lake, Vellore Institute of Technology, Vellore, Tamil Nadu.

Preparation of M. pureins seed extract

The seeds of M. pureins were dried under daylight and were crushed into coarse powder and stored at room temperature. 0.5 g of seeds in 50 mL of MilliQ water was boiled for 45 min at 60 °C. The extract was then cooled, filtered utilizing the Whatman filter paper and stored at 4 °C for further use.

Synthesis of SeNPs

SeNPs were synthesized using the precursor sodium selenite, a ratio of 9:1 was maintained, and 10 mM of the precursor concentration and 1% of seed extract was supplemented. The solution was covered and was kept in the shaker at 150 rpm at room temperature. The synthesized solution was purified using MilliQ water and 70% ethanol by centrifuging at 10,000 rpm for 15 min. The dried samples were procured by lyophilization and stored at room temperature in airtight containers for further purposes.

Characterization of the biosynthesized SeNPs

UV–visible spectroscopy

UV-Vis spectroscopy was utilized to study the bio-reduction of sodium selenite to SeNPs by analyzing the wavelength at which maximum absorbance peak was seen and to decide the time taken for the synthesis. The range was from 300 to 700 nm. The phenomenon explained behind the working principle of UV-Vis is the surface plasmon resonance, which is an optical technique utilized for detecting molecular interactions [18].

Fourier transform infrared spectroscopy (FTIR)

FTIR spectroscopy is considered as an important characterization instrument to portray the structure of nanoparticles. Based on single vibrational modes of various particles, one can uncover its comprising components and its arrangements in their chemical bonds. The FTIR examination was done utilizing Shimadzu Spectroscopy (Model IR Affinity-1) with a wavenumber range of around 400–4000 cm− 1, estimation of the infrared intensity versus the wavenumber gives data about the potential collaborations of the functional groups associated with the bio-reduction procedure [21].

X-ray diffraction (XRD)

XRD offers unmatched precision in the estimation of atomic separating and the X-ray diffractogram of nanomaterials gives an abundance of data from phase composition to crystallite size, from grid strain to crystallographic direction. The essential utilization of powder diffraction is to distinguish its components in the sample [22].

Transmission electron microscopy (TEM)

TEM is the perfect instrument for structural and chemical characterization at the nanoscale. Imaging, diffraction and microanalytical information are quickly produced and then combined to give detailed insights into the properties and behavior of nanostructured materials. It examines the dispersity, the roughness of the particle, in addition to the particle size and its morphology [23].

Dynamic light scattering (DLS)

DLS analysis portrays the transient structure of the molecule’s Brownian motion in fluid suspension, which conveys basic data about the size of the particles and by estimating the transient structure rather than angular distribution, it can quantify particles in nanometers [24].

Scanning electron microscopy (SEM)

SEM is an electron magnifying instrument that examines the sample surface by filtering it with high energy beams of the electron. When the beam of electron strikes the surface of the sample, interacting with the atoms of the sample, secondary electrons are emitted, backscattered electrons and characteristic X-rays are produced that contains data about the sample’s surface topography or its composition. SEM demonstrates three-dimensional images at numerous high amplifications which are nearly up to × 300,000). We utilized ZEISS (EV018) Japan 15 Kv for the examination of the biosynthesized SeNPs [25].

Atomic force microscopy (AFM)

AFM assists in analyzing singular or group of particles and three-dimensional images were produced. The AFM offers perception in three measurements. In the vertical or z-axis, the resolution is constrained by the vibration condition of the instrument: while in the x-y axis, it is characterized by the distance across of tip used for examining. Programming based images prepared can produce quantitative data from individual nanoparticles and between groups of nanoparticles which gives information on the size data and other physical properties (for example, morphology and surface topography) can be estimated [26].

Evaluation of antimicrobial activities of biosynthesised SeNPs

Minimal inhibition concentration (MIC)

MIC of the nanoparticles was found to remove the pathogenic bacterial cultures from water, which was analyzed by the Spread Plate Technique. MIC estimates the lowest level of the compound that is needed to kill or inhibit the growth of bacterial cultures. The ability of the mixture to inhibit the growth of bacteria is based on the extent of its anti-bacterial property. For estimating MIC, a fresh bacterial culture of E. coli was prepared, and then concentrations of the SeNPs formed were made: (50, 250, 500 μg mL− 1). These various sub concentrations were made in different test tubes; nutrient media and bacterial culture were added to it to find out the MIC. The multiple test tubes setups made are shown in Table 1. All the test tubes were incubated for 24 h on a shaker (150 rpm).

Table 1 Various test tubes made to find out the minimum inhibitory concentration

Removal of pathogenic bacterial cultures

The water samples were collected from VIT Lake and to each 100 mL of samples, an optimized dose of SeNPs was added. The samples were then collected aseptically and were then spread on the Nutrient Agar plates at various time intervals (0–60 min). The plates were then incubated for an interval of 24 h at 37 °C, and the plates were analyzed for the growth of bacterial colonies [27].

Dye degradation using biosynthesised SeNPs

Dyes are substances that intensify their interaction with surfaces or textures to give its characteristic color. Based on their electric charge, they are classified into cationic and anionic moieties. They are generally utilized in the manufacturing industries, leather tanning, textile, printing or cosmetic and the utilization of dyes causes contamination in numerous water bodies. Most dyes are destructive to aquatic life since they reduce the penetration of light.

Absorbance readings were taken at various time intervals at a wavelength of 464 nm when SeNPs were treated with multiple groups of Methylene Orange dye, for Coomassie Brilliant Blue, the absorbance was taken at 595 nm and as for Bromophenol Blue (3′,3″,5′,5″-tetrabromophenolsulfonphthalein, BPB), the absorbance was taken at 610 nm and was compared with standard hydrogen peroxide, that is used as a conventional dye absorbent [28].

Statistical analysis

All the results were in triplicate and were expressed as mean ± standard deviation (SD) for the bacterium cultures. The statistical significance between the groups was determined by the one-way ANOVA followed by a test for trial trend and p-value of < 0.05 was considered to be statistically significant.

Results and discussion

Visual coloration and UV-visible analysis

With the addition of seed extract to the precursor, the color of the solution changed into dark brown that indicated the development of SeNPs as shown in Figs. 1 and 2. The occurrence of the color change of the solution is due to the principle of surface plasmon resonance of the SeNPs [29].

Fig. 1
figure 1

The colour change was observed from colourless with the addition of extract and precursor to a dark brown solution when kept for incubation for 8 h

Fig. 2
figure 2

λmax was examined using UV-Vis spectrophotometer of biosynthesised selenium nanoparticles

The pH plays an important role in the synthesis of nanoparticles. In the case of SeNPs, it was optimized by changing the pH which was described by the color change of the reaction mixture and by analyzing the maximum absorbance peak using a UV-Vis spectrophotometer. At pH 6 and below, no color change occurred. It indicates that acidic pH suppresses the nanoparticles synthesis. At pH 7, no color change was observed, which meant that neutral pH does not favor the SeNP synthesis. At pH 8 and 9, the dark brown color was observed after 5 min incubation which turned darker later; hence, pH 8 was the selected pH for the synthesis of SeNPs. Above pH 9, no color change was observed, indicating highly alkaline pH not appropriate for the biosynthesis of nanoparticles. Also, no significant absorbance peak was observed using a spectrophotometer. The differences in the arising of color over the various pH could be due to the presence of different dissociated functional groups of M. pruriens that are involved in the biosynthesis procedure [27, 28].

Wavelength scan for the solution consisting of the M. pruriens along with the sodium selenite was completed in the range from 300 to 700 nm. The characteristic peak was obtained at 319 nm. Agrobacterium-mediated biosynthesis had a λ max at about 300 nm observed by Kora and Rastogi [30], Klebsiella pneumonia synthesized SeNPs had the maximum absorbance at 280 nm by Fesharaki et al. [31], the peak around 261 nm was observed using Diospyros montana as reported by Kokila et al. [32]. The characteristic peak acquired after 8 h showed that the biosynthesis of SeNPs had occurred, the biosynthesis was recorded up to around 10 h; but beyond this time there was a decrease in the peak due to the aggregation of nanoparticles. The broad peak obtained signifies for the spherical-shaped nanoparticles [27]. Lower wavelength is absorbed mainly by particles with smaller size [33].

Electron microscopy

SEM and TEM images show the morphology of the biosynthesized SeNPs using M. pruriens extract. The SEM images reveal that the obtained nanoparticles were spherical shape within a size range of about 100–110 nm and were homogenously dispersed as shown in Figs. 3 and 4. The organic compounds or the biomolecules in the extract are responsible for the reduction and stabilization of the nanoparticles by preventing the aggregation of the nanoparticle [34]. Similar results were obtained for SeNPs synthesized using E. coli ATCC 35218 as reported by Kora and Rastogi [35], chitosan-coated SeNPs also gave uniform spherical morphology reported by Zeng et al. [36] and SeNPs coated with Clausena dentata plant leaf extract reported by Sowndarya et al. [37].

Fig. 3
figure 3

SEM Analysis of the biosynthesised selenium nanoparticles

Fig. 4
figure 4

TEM analysis of the biosynthesised selenium nanoparticles

FTIR analysis

FTIR analysis was carried out to categorize the possible functional groups of the phytochemicals in the seed extract which are accountable for the bio-reduction of the nanoparticles [38]. The FTIR spectrum for the seed extract and SeNPs is demonstrated in Fig. 5; it displays a sharp absorption peak for seed extract at 32694 cm− 1 which designates the occurrence of alcoholic groups, then for biosynthesized SeNPs there was a slight shift in the wavenumber at 3275 cm− 1, other possible peaks for SeNPs were observed at 2912 cm− 1 for aldehyde groups, C-N group for 2147, 1628 and 1603 cm− 1 indicating the presence of C=O stretching vibrations of carbonyls groups. A weak band was observed at 1385 cm− 1 corresponding to the aliphatic bending group. Then at 1231 cm− 1 alkyl ketone group is responsible, then at 1074 cm− 1 alkyl amine groups are present and for the shifted wavenumber 976 cm− 1 the -CH2 group vibrations are present. At 718 cm− 1 falls for the C-H group and at 521 cm− 1 halogen groups are responsible. Similar results were observed by Alagesan and Venugopal [15]. The presence of the alkyl ketone group proves that flavonoids are present as one of the phytochemicals in the seed extract that contains variety of functional groups responsible for the bioreduction. The tautomeric transformation induces the release of hydrogen atoms which results in the conversion to a keto-form from its enol form. The alkyl amine groups indicates the presence of protein in the seed extract containing (−NH2) helps in bioreduction and stabilization of the nanoparticles [39].

Fig. 5
figure 5

FTIR analysis of the biosynthesised selenium nanoparticles

DLS polydispersity index

DLS was utilized for the examination of the homogenous size distribution and to analyze the purity of the biosynthesized SeNPs [40]. The DLS results (Fig. 6) provided the Z-value which was 110.3 nm that is the hydrodynamic radius of the nanoparticles. Also, it offered the polydispersity index value which was 0.3, as it is less than 0.5; which indicates that the nanoparticles formed are homogeneous and well dispersed., similar results were found in size range of 70–360 nm in a study by Avendano et al. [41], chitosan or carboxymethyl coated SeNPs also gave similar results by Chen et al. [42], and between 30 and 150 nm as reported by Sarkar et al. [43].

Fig. 6
figure 6

DLS Analysis of biosynthesised selenium nanoparticles


AFM was done to investigate the morphology, dispersity, porosity and surface topography [44] of the SeNPs. The white patch demonstrated in Fig. 7 portrays the spherical shape of the nanoparticles, while the 3D image gives information on the rough surface topography. From the data, it is interpreted that the average diameter size was around 120 nm with a height of 11 nm. The AFM data gave information on the geometrical characteristics of the nanoparticles.

Fig. 7
figure 7

AFM analysis of the biosynthesised selenium nanoparticles

XRD studies

The crystalline characteristic nature of the biosynthesized SeNPs was evaluated using XRD as shown in Fig. 8. The obtained 2θ values which are characteristic for selenium elements were 23 and 31 at 100, 101 corresponding to crystallographic planes of trigonal selenium, while 2θ value of 26.4 corresponds to 220 crystallographic planes of β-monoclinic selenium [11, 45, 46]. Other peaks (110), (102), (201), (112), (202), (210) correspond to values like 41.8, 44.6, 56.9, 62.9, 65.2 of selenium. Hence, they are in good agreement with the standard JCPDS data (JCPDS file no. 06–0362). Thereby, according to the Debye Scherrer equation [47], the calculated size was 98 nm.

Fig. 8
figure 8

XRD analysis of biosynthesised selenium nanoparticles

Application of SeNPs in dye degradation process

Dyes as well as their by-products are cancer-causing agents or are mutagenic to various living beings and can cause toxic effects to the kidney, liver, brain and central nervous system in individuals [48]. Dye degradation is a methodology where the massive size color atoms are burst down synthetically into smaller size particles. The subsequent by-products are carbon dioxide, water and mineral that imparts the dye color [49]. Methyl Orange is a pH indicator and utilized in titration due to its distinct color at various pH values [50]. BPB is utilized as a pH indicator or dye [51]. Coomassie Brilliant Blue is used in the textile industries and as a staining protein-dye [52].

In the case of Methyl Orange, BPB and Coomassie Brilliant Blue, the absorbance readings, with the setups followed demonstrated in Table 2, were taken using a UV-Vis spectrophotometer at 3 h time interval at 464 nm as shown in Fig. 9.

Table 2 The setups for catalytic dye degradation
Fig. 9
figure 9

Absorbance graph to evaluate the dye degradation of (a) Bromophenol Blue (b) Methyl Orange and (c) Coomassie Brilliant Blue for various setups

As far as the observations are concerned, Setup 4 (Dye + H2O2 + SeNPs) proved to be the most efficient in the case of all the dyes. It indicates that SeNPs are involved in the better degradation of dye as compared to conventional hydrogen peroxide which requires a catalyst to act. It could also be considered that SeNPs act as the catalyst for hydrogen peroxide and together they both help in efficient dye degradation. But the various dyes used as the model dyes in the experiment took different time to reach the constant concentration after the degradation. It is because the structures of all the dyes are different [53]. Methyl Orange has the simplest structure out of the others, hence took the least time to get degraded and achieve its constant concentration. Coomassie Brilliant Blue has the most complex structure; therefore, the time required to get dye degraded is more than those of Methyl Orange and BPB. The mechanism of catalytic degradation is represented through Eqs. (1), (2) and (3)

$$ {\mathrm{H}}_2{\mathrm{O}}_2+\mathrm{e}={\mathrm{O}\mathrm{H}}^{-}+\mathrm{OH}\to $$
$$ \mathrm{Dye}\ \left(\mathrm{Methyl}\ \mathrm{Orange},\kern0.5em \mathrm{Coomassie}\ \mathrm{Brilliant}\ \mathrm{Blue}\ \mathrm{and}\ \mathrm{BPB}\right)+\mathrm{SeNPs}\ast ={\mathrm{SeNPs}}^{-}+{\mathrm{dye}}^{+}\to $$
$$ \mathrm{Dye}+\mathrm{SeNPs}\ast +{\mathrm{H}}_2{\mathrm{O}}_2={\mathrm{SeNPs}}^{-}+\mathrm{degraded}\ \mathrm{product}+{\mathrm{CO}}_2+{\mathrm{H}}_2\mathrm{O}\to $$

Application of SeNPs in removing bacteria from the water

MIC analysis was performed and similar results were observed [54] and the MIC value was added to lake water and was spread onto the Nutrient Agar Plate. It is very clear from the spread plates that at time t = 0 min, maximum bacterial growth is observed, but at the time t = 60 min, the bacterial growth was minimum as shown in Figs. 10 and 11. Hence, SeNPs could be considered as the way of purifying the water by killing or inhibiting the growth of the pathogenic bacterial cultures. The production of safe drinking water is a significant obstacle in both developed and developing countries. The demand for better water quality management has led to the innovation of disinfectants; amongst these SeNPs can also be a part of it. Many nanoparticles have been used in the past years for water purification. Silver nanoparticles help remove pesticides and halogenated organics, Titania nanoparticles removes arsenic and also disinfect the water, Iron oxide nanoparticles remove heavy metals including arsenic, lead, chromium, zinc, copper and ceramic nanopowder also is used for disinfection of water [55,56,57,58,59].

Fig. 10
figure 10

Pathogenic growth inhibition when biosynthesised SeNPs are treated with lake water

Fig. 11
figure 11

Antimicrobial efficiency of biosynthesized SeNPs on removal of pathogens from lake water


The biosynthesis of SeNPs mediated through a green route using seed extract of M. pruriens at physiological conditions like room temperature, is a procedure which is simple, eco- friendly and highly efficient. The time intervals for the reaction with aqueous sodium selenite solution showed an increase in the absorbance with time and became constant giving a maximum absorbance at 319.4 nm after an incubation of 8 h. The results from DLS, XRD, SEM and TEM suggested the biosynthesis SeNP to be spherical with a size of 95–110 nm. FTIR indicates the presence of alcoholic groups with C=O bonds and C–O stretching of carboxylic groups. The synthesized nanoparticles exhibit dye degradation activity indicating that SeNPs are involved in better degradation of dye as compared to the conventional hydrogen peroxide which requires a catalyst to act. The synthesized SeNPs also shows pathogenic bacterial culture removal activity from an open-water source. The optimum concentration for removal of the pathogenic bacterial culture was found to be 250 μg mL− 1. The optimum time for the removal process was 60 min. Consequently, it can be concluded that the biosynthesized SeNPs have an effective capacity in pathogenic bacterial culture removal and can be used for purification of contaminated water.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.


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The authors would like to thank the Vellore Institute of Technology for all the resources and funding to proceed with the study.


The authors certify that they have no affiliations with or involvement in any organization or entity with any financial interestor non-financial interest in the subject matter or materials discussed in this manuscript.


The funding was sanctioned by the Vellore Institute of Technology through seed grants.

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SM carried out the design of research, instrumental analysis interpretation of nanomaterials, and writing the manuscript. HA and VKS participated in the correction of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Venkat Kumar Shanmugam.

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Menon, S., Agarwal, H. & Shanmugam, V.K. Catalytical degradation of industrial dyes using biosynthesized selenium nanoparticles and evaluating its antimicrobial activities. Sustain Environ Res 31, 2 (2021).

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